超微量分光光度計(jì)區(qū)別于傳統(tǒng)分光光度計(jì)的優(yōu)勢(shì)特點(diǎn)

更新時(shí)間：2022-04-14

點(diǎn)擊次數(shù)：1208

　　超微量分光光度計(jì)近年來已經(jīng)替換普通的分光光度計(jì)成為分子生物學(xué)實(shí)驗(yàn)室的新寵，廣泛應(yīng)用于生命科學(xué)實(shí)驗(yàn)室蛋白質(zhì)組學(xué)和基因組學(xué)等領(lǐng)域。

　　分光光度計(jì)就是利用分光光度法對(duì)物質(zhì)進(jìn)行定量定性分析的儀器。由于核酸，蛋白定量以及細(xì)菌生長(zhǎng)濃度的定量樣本量很小，于是超微量光度計(jì)應(yīng)運(yùn)而生。

　　超微量分光光度計(jì)與傳統(tǒng)分光光度計(jì)相比，前者具備的*優(yōu)點(diǎn)在于：

　　1、所需樣品體積小，僅需0.5—2μl；

　　2、不需要比色皿，用移液槍直接將樣品滴加到檢測(cè)平臺(tái)上，測(cè)量時(shí)樣品自動(dòng)形成液柱，檢測(cè)完成后只需用干凈的吸水紙將樣品從檢測(cè)平臺(tái)上擦拭干凈即可，避免了因?yàn)楸壬笄逑床粌魩淼慕徊嫖廴荆?/div>

　　3、一般具有多個(gè)光程（電機(jī)控制自動(dòng)選擇），基座模式下光程1.0mm、0.2mm、0.1mm和0.05mm自動(dòng)調(diào)整，而傳統(tǒng)分光光度計(jì)的光程為10mm，超微量分光光度計(jì)樣品無需稀釋，測(cè)量范圍可達(dá)到常規(guī)分光光度計(jì)的50倍；

　　4、氙氣閃光燈為燈源，代替了氘燈（紫外）和鎢燈（可見），使用壽命更長(zhǎng)；

　　5、不需要預(yù)熱，可隨時(shí)檢測(cè)，檢測(cè)時(shí)間短；

　　6、顯示吸光度值的同時(shí)，程序直接給出濃度值（核酸、蛋白和熒光染料）；

　　7、占據(jù)實(shí)驗(yàn)室空間體積比傳統(tǒng)分光光度計(jì)小很多。

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